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KMID : 0895420130230020050
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Journal of Korean Society of Occupational and Enviromental Hygiene
2013 Volume.23 No. 2 p.50 ~ p.56
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Cytotoxicity and DNA Damage in Human Lung Cells Treated with Lanthanum Oxide and Neodymium Oxide
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Kim Jong-Kyu
Kim Soo-Jin
Kang Min-Gu
Chung Yong-Hyun
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Abstract
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Objectives: The present study investigated cytotoxicity and DNA damage in human lung cells in vitro.
Methods: Neodymium oxide and lanthanum oxide were dispersed by ultrasonic treatments. The assay was performed with MRC-5 (Human male fetus lung cell). Cytotoxicity and comet assay of lanthanum oxide and neodymium oxide were measured after 24 and 48 hours incubation.
Results: After 24 hours of exposure to rare earth metals, the cytotoxicities of lanthanum oxide in more than 1μM concentration groups were significantly increased when compared to the control group, but the cytotoxicities of neodymiun oxide in more than 100 μM concentration groups were statistically increased. After 48 hours exposure, cytotoxicities of both materials were statistically increased in 100,000 μM concentration groups. Olive tail moments of the lanthanum oxide treated group were significantly increased when compared to the control group.
Conclusions: The cytotoxicity of lanthanum oxide was higher than that of neodymium oxide. The DNA of MRC-5 cells treated with lanthanum oxide for 48 hours were significantly damaged.
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KEYWORD
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rare earth metals(REM), cell toxicity, Comet assay, MRC-5
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